Hotspot

rpoB-trnC-GCA

LSC · 28443-29588 · 1146 bp

Back to Ficus

Pi 0.0118
MarkerSeek score 62.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1146
variable_sites
94
pi
0.0118
unique_haplotype_count
38
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTTCCTCATTTCCACCCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.4 58.1 1192-1242 bp 0.97561 84.2
rpoB-trnC-GCA_p2 ATTTCCTCATTTCCACCCCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.4 58.4 1174-1224 bp 0.97561 82.1
rpoB-trnC-GCA_p3 TTCCTCATTTCCACCCCCAA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.8 58.1 1190-1240 bp 0.97561 81.9
rpoB-trnC-GCA_p4 TTTCCTCATTTCCACCCCCA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.8 58.1 1191-1241 bp 0.97561 81.9
rpoB-trnC-GCA_p5 ATTTCCTCATTTCCACCCCC TATTGATTAGGCGACACCCG 50.0 50.0 57.4 57.5 1209-1259 bp 0.97561 80.6