Hotspot

rpoC2

LSC · 16840-20994 · 4155 bp

Back to Ficaria

Pi 0.000481

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4155
variable_sites: 2
pi: 0.000481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ATTCAAGGTGGATGCTTCGG	CCGGTGGAGTATTTACAGGC	50.0	55.0	58.2	58.1	275 bp	1.0	78.2
rpoC2_p2	ATTCAAGGTGGATGCTTCGG	GGTGGAGTATTTACAGGCGG	50.0	55.0	58.2	58.1	273 bp	1.0	78.2
rpoC2_p3	ATTCAAGGTGGATGCTTCGG	GGCGGTACTGAAGAACATGT	50.0	50.0	58.2	57.9	258 bp	1.0	77.9
rpoC2_p4	ATTCAAGGTGGATGCTTCGG	AGGCGGTACTGAAGAACATG	50.0	50.0	58.2	57.6	259 bp	1.0	75.6
rpoC2_p5	ATTCAAGGTGGATGCTTCGG	CAGGCGGTACTGAAGAACAT	50.0	50.0	58.2	57.6	260 bp	1.0	75.6