Hotspot

accD

LSC · 59341-60819 · 1479 bp

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Pi 0

MarkerSeek score 26.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCCCCTCCGTATATTGTCCT	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	2365 bp	1.0	80.1
accD_p2	TGTCCTTTTCGTTCCGTGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	2351 bp	1.0	80.0
accD_p3	TTGTCCTTTTCGTTCCGTGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	2352 bp	1.0	80.0
accD_p4	GGATCAAACCAAGGAGCACA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2396 bp	1.0	79.5
accD_p5	TGGATCAAACCAAGGAGCAC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2397 bp	1.0	79.5