Hotspot

ccsA-ndhD

SSC · 123927-124131 · 205 bp

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Pi 0.0341

MarkerSeek score 72.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 205
variable_sites: 7
pi: 0.0341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCAAATTCCGCAATTGTGGC	TCCGGATTTTGTTCTCTCACT	45.0	42.9	57.9	57.0	395-404 bp	1.0	64.4
ccsA-ndhD_p2	CAAATTCCGCAATTGTGGCT	TCCGGATTTTGTTCTCTCACT	45.0	42.9	57.9	57.0	394-403 bp	1.0	64.3
ccsA-ndhD_p3	TTCCGCAATTGTGGCTTCTA	TCCGGATTTTGTTCTCTCACT	45.0	42.9	57.8	57.0	390-399 bp	1.0	63.5
ccsA-ndhD_p4	TCAAATTCCGCAATTGTGGC	TCTACCCGTGATAGGTATTGGT	45.0	45.5	57.9	57.8	422-431 bp	1.0	63.2
ccsA-ndhD_p5	CAAATTCCGCAATTGTGGCT	TCTACCCGTGATAGGTATTGGT	45.0	45.5	57.9	57.8	421-430 bp	1.0	63.1