Hotspot

accD-psaI

LSC · 60820-61243 · 424 bp

Back to Ficaria

Pi 0.0283

MarkerSeek score 71.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 12
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTTCACGGTTTCTTTCCA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.7	58.0	538 bp	1.0	78.8
accD-psaI_p2	AGCTTCACGGTTTCTTTCCA	TGCCGGAAATACTAGACCCA	45.0	50.0	57.7	58.1	528 bp	1.0	78.0
accD-psaI_p3	AGCTTCACGGTTTCTTTCCA	GCCGGAAATACTAGACCCAC	45.0	55.0	57.7	57.8	527 bp	1.0	77.2
accD-psaI_p4	AGCTTCACGGTTTCTTTCCA	GAGAAGCCATTGCAATTGCC	45.0	50.0	57.7	58.4	544 bp	1.0	76.2
accD-psaI_p5	CAGCTTCACGGTTTCTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	539 bp	1.0	76.1