Hotspot

rbcL-accD

LSC · 58649-59340 · 692 bp

Back to Ficaria

Pi 0.00289
MarkerSeek score 60.60
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
692
variable_sites
2
pi
0.00289
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TGCAAATGGAGCCTTGAACT GACGGGACCCATACCTTCTA 45.0 55.0 58.0 57.9 868-869 bp 1.0 79.7
rbcL-accD_p2 TGCAAATGGAGCCTTGAACT ACGGGACCCATACCTTCTAG 45.0 55.0 58.0 57.9 867-868 bp 1.0 79.6
rbcL-accD_p3 TGCAAATGGAGCCTTGAACT CGGGACCCATACCTTCTAGT 45.0 55.0 58.0 57.9 866-867 bp 1.0 79.6
rbcL-accD_p4 TGCAAATGGAGCCTTGAACT CACACCTGTGCTCTAACTCC 45.0 55.0 58.0 57.9 833-834 bp 1.0 79.6
rbcL-accD_p5 TGCAAATGGAGCCTTGAACT CCACACCTGTGCTCTAACTC 45.0 55.0 58.0 57.9 834-835 bp 1.0 79.6