Hotspot

atpB-rbcL

LSC · 56452-57220 · 769 bp

Back to Ficaria

Pi 0.0013

MarkerSeek score 59.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 1
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	GCCGCCAAGGTATCAGTATC	55.0	55.0	58.0	58.2	982 bp	1.0	78.1
atpB-rbcL_p2	TTTTCTTCAAGCGCGGAAAC	GCCGCCAAGGTATCAGTATC	45.0	55.0	57.9	58.2	938 bp	1.0	77.6
atpB-rbcL_p3	ATCCAGTACCGGACCAATGA	GCCGCCAAGGTATCAGTATC	50.0	55.0	58.1	58.2	981 bp	1.0	77.5
atpB-rbcL_p4	TCCAGTACCGGACCAATGAT	GCCGCCAAGGTATCAGTATC	50.0	55.0	58.1	58.2	980 bp	1.0	77.5
atpB-rbcL_p5	AAACCCCAGGACCAGAAGTA	GCCGCCAAGGTATCAGTATC	50.0	55.0	57.9	58.2	922 bp	1.0	77.4