Hotspot

ndhC-trnV-UAC

LSC · 52528-53404 · 877 bp

Back to Ficaria

Pi 0.00342
MarkerSeek score 60.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
877
variable_sites
3
pi
0.00342
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCGGCCCTTCATTAATTGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1026 bp 1.0 78.1
ndhC-trnV-UAC_p2 CCCTTCATTAATTGGGGCCA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1021 bp 1.0 77.3
ndhC-trnV-UAC_p3 TCCGGCCCTTCATTAATTGG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1025 bp 1.0 75.4
ndhC-trnV-UAC_p4 TAATTGGGGCCAAAACTCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1013 bp 1.0 75.1
ndhC-trnV-UAC_p5 CCCTTCATTAATTGGGGCCA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.8 57.5 1020 bp 1.0 74.5