Hotspot

petN-psbM

LSC · 29211-30426 · 1216 bp

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Pi 0.000823
MarkerSeek score 59.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1216
variable_sites
1
pi
0.000823
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTGTTCATTCT 45.0 42.9 58.0 57.6 1370-1371 bp 1.0 68.8
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTGTTCATTCT 50.0 42.9 57.8 57.6 1376-1377 bp 1.0 67.3
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1316-1317 bp 1.0 66.1
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTGTTCATTC 45.0 45.0 58.0 56.1 1370-1371 bp 1.0 64.6
petN-psbM_p5 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTGTTCATTC 50.0 45.0 57.8 56.1 1376-1377 bp 1.0 63.1