Hotspot

rpl32-trnL-UAG

SSC · 103629-104085 · 457 bp

Back to Festuca

Pi 0.0321

MarkerSeek score 69.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 457
variable_sites: 59
pi: 0.0321
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAATGAGCATCCAAAACCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.3	58.0	588-837 bp	1.0	85.9
rpl32-trnL-UAG_p2	AGGGAATAGGCTTCCGTACC	TTCGAACCGAGATGCTTGAG	55.0	50.0	58.6	58.0	1051-1289 bp	0.941176	83.6
rpl32-trnL-UAG_p3	AGGGAATAGGCTTCCGTACC	GGATTCGAACCGAGATGCTT	55.0	50.0	58.6	58.1	1054-1292 bp	0.941176	83.1
rpl32-trnL-UAG_p4	AGGGAATAGGCTTCCGTACC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.6	57.9	1024-1262 bp	0.941176	82.8
rpl32-trnL-UAG_p5	AGGGAATAGGCTTCCGTACC	GATCCGCACAAAAGAGTTGC	55.0	50.0	58.6	58.0	1603-1841 bp	0.941176	81.2