Hotspot

ndhF-rpl32

SSC · 102571-103436 · 866 bp

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Pi 0.0174

MarkerSeek score 68.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 866
variable_sites: 65
pi: 0.0174
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TTCGAACCGAGATGCTTGAG	55.0	50.0	58.1	58.0	1572-1926 bp	1.0	89.4
ndhF-rpl32_p2	GAAGAGGGAATTGTGCGGAT	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	2261-2615 bp	1.0	88.5
ndhF-rpl32_p3	GAAGAGGGAATTGTGCGGAT	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	2264-2618 bp	1.0	88.0
ndhF-rpl32_p4	GAAGAGGGAATTGTGCGGAT	GGTTTTGGATGCTCATTGCC	50.0	50.0	58.0	58.0	1656-1814 bp	1.0	87.2
ndhF-rpl32_p5	GAAGAGGGAATTGTGCGGAT	ATGCTCATTGCCGCTAGAAA	50.0	45.0	58.0	58.0	1648-1806 bp	0.941176	83.8