Hotspot

rpl22-rps19

LSC · 79011-79088 · 78 bp

Back to Festuca

Pi 0.0498

MarkerSeek score 70.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 78
variable_sites: 11
pi: 0.0498
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CCTAGCGAGTTTCCTTAGCC	GGAATTCGTACCTACTCGGC	55.0	55.0	57.8	57.9	223-224 bp	1.0	82.2
rpl22-rps19_p2	CTAGCGAGTTTCCTTAGCCC	GGAATTCGTACCTACTCGGC	55.0	55.0	57.8	57.9	222-223 bp	1.0	82.2
rpl22-rps19_p3	CCTAGCGAGTTTCCTTAGCC	ATGGTAGGTCGCAAATTGGG	55.0	50.0	57.8	58.2	243-244 bp	1.0	80.9
rpl22-rps19_p4	CTAGCGAGTTTCCTTAGCCC	ATGGTAGGTCGCAAATTGGG	55.0	50.0	57.8	58.2	242-243 bp	1.0	80.9
rpl22-rps19_p5	CTAGCGAGTTTCCTTAGCCC	GTCGCAAATTGGGGGAATTC	55.0	50.0	57.8	57.7	235-236 bp	1.0	80.6