Hotspot

petA-psbJ

LSC · 59478-60104 · 627 bp

Back to Festuca

Pi 0.0176

MarkerSeek score 57.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 36
pi: 0.0176
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTAAAGAAAAAGGCGGGT	CCCATTTGGAAGGATACCCG	45.0	55.0	58.0	58.0	1153-1305 bp	1.0	85.0
petA-psbJ_p2	AAACCGAGGAAGAGGACAGA	CCCATTTGGAAGGATACCCG	50.0	55.0	58.0	58.0	1246-1398 bp	1.0	84.8
petA-psbJ_p3	AAACCGAGGAAGAGGACAGA	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1705-1857 bp	1.0	83.9
petA-psbJ_p4	ACGTAAAGAAAAAGGCGGGT	TGCGGGTTGGTTATTTGTCA	45.0	45.0	58.0	58.0	1612-1764 bp	1.0	83.9
petA-psbJ_p5	CTTGTGATAGGCCCTGTTCC	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1824-1976 bp	1.0	83.4