Hotspot

rbcL-psaI

LSC · 55006-56182 · 1177 bp

Back to Festuca

Pi 0.0128

MarkerSeek score 54.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 62
pi: 0.0128
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GCTTCGGGTGGTATTCATGT	CCAAGATAACTGGAAGCCCC	50.0	55.0	58.0	57.9	1755-2116 bp	1.0	86.9
rbcL-psaI_p2	CATAACTGGGGGATTCACCG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	2080-2441 bp	1.0	86.7
rbcL-psaI_p3	TTTTTCACTCAGGACTGGGC	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1570-1645 bp	0.647059	69.4
rbcL-psaI_p4	TTTTTCACTCAGGACTGGGC	CCAAGATAACTGGAAGCCCC	50.0	55.0	58.0	57.9	2056-2131 bp	0.647059	69.1
rbcL-psaI_p5	TTTTTCACTCAGGACTGGGC	CATACGTGTTCTGATCGCCA	50.0	50.0	58.0	58.1	1948-2023 bp	0.647059	69.1