Hotspot

trnL-UAA-trnF-GAA

LSC · 46242-46582 · 341 bp

Back to Festuca

Pi 0.0216

MarkerSeek score 64.00

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 25
pi: 0.0216
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	431-458 bp	1.0	81.9
trnL-UAA-trnF-GAA_p2	AAGTCGTGAGGGTTCAAGTC	TCTGCCAGGAACCAGATTTG	50.0	50.0	57.5	57.8	447-474 bp	1.0	80.7
trnL-UAA-trnF-GAA_p3	AAGTCGTGAGGGTTCAAGTC	CTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	57.8	446-473 bp	1.0	80.5
trnL-UAA-trnF-GAA_p4	AAGTCGTGAGGGTTCAAGTC	TTTTCTCTGCCAGGAACCAG	50.0	50.0	57.5	57.7	452-479 bp	1.0	80.4
trnL-UAA-trnF-GAA_p5	AAGTCGTGAGGGTTCAAGTC	TCTCTGCCAGGAACCAGATT	50.0	50.0	57.5	58.3	449-476 bp	1.0	79.9