Hotspot

petN-trnC-GCA

LSC · 16625-17527 · 903 bp

Back to Festuca

Pi 0.0132

MarkerSeek score 62.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 51
pi: 0.0132
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	TTTCTCGATTTTCGCTCGGA	50.0	45.0	58.2	57.6	1027-1047 bp	1.0	80.9
petN-trnC-GCA_p2	CCACTCCTCCCCCATACTAC	TTTCTCGATTTTCGCTCGGA	60.0	45.0	58.3	57.6	1083-1103 bp	1.0	79.7
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	TCTCGATTTTCGCTCGGATT	50.0	45.0	58.2	57.4	1025-1045 bp	1.0	79.2
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	TTCTCGATTTTCGCTCGGAT	50.0	45.0	58.2	57.4	1026-1046 bp	1.0	79.1
petN-trnC-GCA_p5	TCCACTCCTCCCCCATACTA	TTTCTCGATTTTCGCTCGGA	55.0	45.0	58.4	57.6	1084-1104 bp	1.0	79.0