Hotspot

trnD-GUC-psbM

LSC · 15453-16149 · 697 bp

Back to Festuca

Pi 0.00806

MarkerSeek score 60.30

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 31
pi: 0.00806
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	50.0	45.0	58.5	55.8	591-818 bp	1.0	65.5
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	AGAATGAACAGTGCAGTAGC	55.0	45.0	58.7	55.8	623-850 bp	1.0	64.0
trnD-GUC-psbM_p3	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.0	55.8	592-819 bp	1.0	61.3
trnD-GUC-psbM_p4	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.9	55.8	605-832 bp	1.0	60.1
trnD-GUC-psbM_p5	TCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.8	55.8	590-817 bp	1.0	59.5