Hotspot

psbK-psbI

LSC · 6968-7377 · 410 bp

Back to Festuca

Pi 0.0153

MarkerSeek score 60.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 19
pi: 0.0153
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	50.0	50.0	57.9	57.8	508-538 bp	1.0	84.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	GAATTTTTACTCCTCGCGCC	50.0	50.0	57.9	57.5	533-563 bp	1.0	83.0
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	GGAATTTTTACTCCTCGCGC	50.0	50.0	57.9	57.5	534-564 bp	1.0	83.0
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TCCGTCCTGGGTCATTAGAT	50.0	50.0	57.9	57.5	507-537 bp	1.0	83.0
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TTCCGTCCTGGGTCATTAGA	45.0	50.0	58.3	57.8	522-535 bp	0.941176	79.0