Hotspot

ycf1

SSC · 131102-136582 · 5481 bp

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Pi 0.00461
MarkerSeek score 51.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5481
variable_sites
260
pi
0.00461
unique_haplotype_count
31
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGTTGTGTAGGAATTCTGCCA TCTGACTGATAGAACAAGCACA 42.9 40.9 57.2 57.1 270 bp 0.941176 45.7
ycf1_p2 AGTTGTGTAGGAATTCTGCCAA TCTGACTGATAGAACAAGCACA 40.9 40.9 57.9 57.1 270 bp 0.970588 44.7
ycf1_p3 AGGAGTTGTGTAGGAATTCTGC TCTGACTGATAGAACAAGCACA 45.5 40.9 58.1 57.1 273 bp 0.941176 43.8
ycf1_p4 AGTTGTGTAGGAATTCTGCCA TCTGACTGATAGAACAAGCACAA 42.9 39.1 57.2 57.8 270 bp 0.941176 43.0
ycf1_p5 AGTTGTGTAGGAATTCTGCCA CTGACTGATAGAACAAGCACA 42.9 42.9 57.2 55.6 269 bp 0.941176 43.0