Hotspot

rpl22

LSC · 84501-84995 · 495 bp

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Pi 0.00356
MarkerSeek score 39.60
Primer available yes
Label rpl22

Feature Metadata

label_name
rpl22
feature_type
gene
region
LSC
length_bp
495
variable_sites
20
pi
0.00356
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22_p1 CCAAGTCTGAAACCAAGTGGA TCATTCATTAGTAGGAGGCA 47.6 40.0 58.1 53.1 549 bp 0.970588 41.9
rpl22_p2 CCAAGTCTGAAACCAAGTGGA TCATTCATTAGTAGGAGGCAA 47.6 38.1 58.1 54.0 549 bp 0.970588 41.9
rpl22_p3 ACCAAGTCTGAAACCAAGTGG TCATTCATTAGTAGGAGGCA 47.6 40.0 58.3 53.1 550 bp 0.970588 41.9
rpl22_p4 CCAAGTCTGAAACCAAGTGGA ATCATTCATTAGTAGGAGGCA 47.6 38.1 58.1 53.8 550 bp 0.970588 41.9
rpl22_p5 CCAAGTCTGAAACCAAGTGGA GCTTATCATTCATTAGTAGGAGGC 47.6 41.7 58.1 57.0 554 bp 0.941176 40.5