Hotspot

psbE-petL

LSC · 65821-66985 · 1165 bp

Back to Ferula

Pi 0.00375
MarkerSeek score 64.90
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1165
variable_sites
49
pi
0.00375
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1110-1324 bp 1.0 86.7
psbE-petL_p2 CAACCCGCAATGAATAGGGA TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1113-1327 bp 1.0 86.1
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.8 58.0 1033-1247 bp 1.0 85.9
psbE-petL_p4 ACTGACGAATAACCAACCCG GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1123-1337 bp 0.941176 83.7
psbE-petL_p5 ACTGACGAATAACCAACCCG TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1126-1340 bp 0.941176 83.1