Hotspot

petA-psbJ

LSC · 64041-65044 · 1004 bp

Back to Ferula

Pi 0.00666
MarkerSeek score 56.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1004
variable_sites
49
pi
0.00666
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTTACGTGTCCAAGGCCTTT CTGGATTGGGTTCATCTCTGT 50.0 47.6 58.1 57.4 1097-1206 bp 0.970588 72.5
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC CTGGATTGGGTTCATCTCTGT 50.0 47.6 57.8 57.4 1084-1193 bp 0.970588 72.1
petA-psbJ_p3 GATCCCTTACGTGTCCAAGG CTGGATTGGGTTCATCTCTGT 55.0 47.6 57.7 57.4 1102-1211 bp 0.970588 70.8
petA-psbJ_p4 CTTACGTGTCCAAGGCCTTT TGGATTGGGTTCATCTCTGT 50.0 45.0 58.1 56.1 1096-1205 bp 0.970588 70.6
petA-psbJ_p5 TTACGTGTCCAAGGCCTTTT CTGGATTGGGTTCATCTCTGT 45.0 47.6 57.6 57.4 1096-1205 bp 0.970588 70.5