Hotspot

ycf4-cemA

LSC · 61442-62141 · 700 bp

Back to Ferula

Pi 0.00448
MarkerSeek score 64.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
700
variable_sites
33
pi
0.00448
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 AATCGAACAAAAAGCTGCCG GAGAAATCCACCAGGGCAAA 45.0 50.0 57.9 58.1 662-843 bp 1.0 86.3
ycf4-cemA_p2 CGAACAAAAAGCTGCCGAAT GAGAAATCCACCAGGGCAAA 45.0 50.0 57.9 58.1 659-840 bp 1.0 86.3
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT GAGAAATCCACCAGGGCAAA 45.0 50.0 58.1 58.1 640-821 bp 1.0 85.9
ycf4-cemA_p4 TTGCGTGTACCGATTGAAGT GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 628-809 bp 1.0 85.5
ycf4-cemA_p5 ACAAAAAGCTGCCGAATTGG GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 656-837 bp 1.0 85.4