Hotspot

trnT-GGU-psbD

LSC · 32336-33754 · 1419 bp

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Pi 0.00694
MarkerSeek score 56.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1419
variable_sites
101
pi
0.00694
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AAGGGCCCTTTTAACTCAGC CCAGCCTCCTAAAGCGAAAT 50.0 50.0 58.1 58.0 1630-1656 bp 1.0 88.1
trnT-GGU-psbD_p2 AAGGGCCCTTTTAACTCAGC CCAGAAAACGCGATTGCATT 50.0 45.0 58.1 58.0 1929-1955 bp 1.0 87.1
trnT-GGU-psbD_p3 AAGGGCCCTTTTAACTCAGC AACTAGGCGCAAAGAACCAA 50.0 45.0 58.1 58.0 2003-2029 bp 1.0 86.8
trnT-GGU-psbD_p4 AAGGGCCCTTTTAACTCAGC AAACTAGGCGCAAAGAACCA 50.0 45.0 58.1 58.0 2004-2030 bp 1.0 86.7
trnT-GGU-psbD_p5 AAGGGCCCTTTTAACTCAGC ACCAAAACTAGGCGCAAAGA 50.0 45.0 58.1 58.0 2008-2034 bp 1.0 86.7