Hotspot

atpH-atpI

LSC · 13881-15051 · 1171 bp

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Pi 0.00455
MarkerSeek score 67.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1171
variable_sites
61
pi
0.00455
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT ATAGGGGAATCCATGGAGGG 45.0 55.0 58.0 57.9 1045-1255 bp 1.0 87.2
atpH-atpI_p2 TACCTTGACCAACTCCAGGT ATAGGGGAATCCATGGAGGG 50.0 55.0 57.9 57.9 1101-1311 bp 1.0 86.4
atpH-atpI_p3 AGCCAATCCAGCAGCAATAA ATAGGGGAATCCATGGAGGG 45.0 55.0 57.9 57.9 1061-1271 bp 1.0 86.3
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA ATAGGGGAATCCATGGAGGG 45.0 55.0 58.2 57.9 1046-1256 bp 1.0 86.0
atpH-atpI_p5 GCAGTACCTTGACCAACTCC ATAGGGGAATCCATGGAGGG 55.0 55.0 58.2 57.9 1105-1315 bp 1.0 85.8