Hotspot

trnS-GCU-trnG-UCC

LSC · 8750-9307 · 558 bp

Back to Ferula

Pi 0.00539
MarkerSeek score 64.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
558
variable_sites
35
pi
0.00539
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA GGGGGTTACTCGAACGAATC 45.0 55.0 58.0 58.1 632-667 bp 1.0 87.6
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA GGGGGTTACTCGAACGAATC 50.0 55.0 58.2 58.1 681-716 bp 1.0 86.4
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA AGGGGGTTACTCGAACGAAT 45.0 50.0 58.0 58.4 633-668 bp 1.0 84.7
trnS-GCU-trnG-UCC_p4 GCTTTAGTCCACTCAGCCAT GGGGGTTACTCGAACGAATC 50.0 55.0 57.6 58.1 618-653 bp 1.0 84.7
trnS-GCU-trnG-UCC_p5 AGTCCACTCAGCCATCTCTC GGGGGTTACTCGAACGAATC 55.0 55.0 58.5 58.1 613-648 bp 1.0 83.9