Hotspot

trnH-GUG-psbA

LSC · 77-263 · 187 bp

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Pi 0.0109
MarkerSeek score 64.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
187
variable_sites
21
pi
0.0109
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 293-326 bp 1.0 86.6
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 306-339 bp 1.0 86.3
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 307-340 bp 1.0 86.2
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 308-341 bp 1.0 86.2
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG ATCGCGCTAACCTTGGTATG 55.0 50.0 58.1 58.1 296-329 bp 1.0 85.7