Hotspot

rps15

SSC · 123483-123749 · 267 bp

Back to Farsetia

Pi 0.0112

MarkerSeek score 44.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 267
variable_sites: 3
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TCTGTGCACCAATATCTGCC	TCCCCTAAACGACGTAGAGA	50.0	50.0	58.0	57.2	1385 bp	1.0	74.0
rps15_p2	GCTGGCCCTTTTAGGAACTT	TCCCCTAAACGACGTAGAGA	50.0	50.0	58.1	57.2	1297 bp	1.0	73.8
rps15_p3	TCCCCACCAATAATGCCAAC	TCCCCTAAACGACGTAGAGA	50.0	50.0	58.1	57.2	1635 bp	1.0	73.2
rps15_p4	CTTCCCCACCAATAATGCCA	TCCCCTAAACGACGTAGAGA	50.0	50.0	57.8	57.2	1637 bp	1.0	73.0
rps15_p5	CACCAATAATGCCAACCCCT	TCCCCTAAACGACGTAGAGA	50.0	50.0	58.1	57.2	1631 bp	0.5	52.6