Hotspot

psbA

LSC · 362-1423 · 1062 bp

Back to Farsetia

Pi 0.00282

MarkerSeek score 50.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTAGGTCTAGACTAACGCT	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	54.4	58.4	1138 bp	1.0	48.6
psbA_p2	AACTAGGTCTAGACTAACGCT	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	55.3	58.4	1139 bp	1.0	47.4
psbA_p3	AGAAGGCTTATATTGCTCGT	ATTTGTGTGCTTGGGAGTCC	40.0	50.0	54.2	58.4	1174 bp	1.0	47.1
psbA_p4	AAGAAGGCTTATATTGCTCGT	ATTTGTGTGCTTGGGAGTCC	38.1	50.0	55.1	58.4	1175 bp	1.0	45.9
psbA_p5	AGAAGGCTTATATTGCTCGTT	ATTTGTGTGCTTGGGAGTCC	38.1	50.0	55.1	58.4	1174 bp	1.0	45.9