Hotspot

rpl32-trnL-CUA

SSC · 113676-114432 · 757 bp

Back to Farsetia

Pi 0.0225

MarkerSeek score 58.90

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 757
variable_sites: 17
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	CTCGAAAAAGCGCATTCGTA	TGCAGACTGAGGCATTTTGT	45.0	45.0	57.2	58.0	1462-1514 bp	1.0	74.7
rpl32-trnL-CUA_p2	CTCGAAAAAGCGCATTCGTA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.2	57.9	926-978 bp	1.0	74.4
rpl32-trnL-CUA_p3	CTCGAAAAAGCGCATTCGTA	TAACCACTGGGACTGGAGAG	45.0	55.0	57.2	58.1	1495-1547 bp	1.0	74.3
rpl32-trnL-CUA_p4	TCTCGAAAAAGCGCATTCGT	TGCAGACTGAGGCATTTTGT	45.0	45.0	58.9	58.0	1463-1515 bp	1.0	74.1
rpl32-trnL-CUA_p5	TCTCGAAAAAGCGCATTCGT	TAACCACTGGGACTGGAGAG	45.0	55.0	58.9	58.1	1496-1548 bp	1.0	73.7