Hotspot

rps8-rpl14

LSC · 80180-80434 · 255 bp

Back to Farsetia

Pi 0.0196

MarkerSeek score 55.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 255
variable_sites: 5
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGATCCTATCCGAACCGTTC	CCCACGGGAATTGAGACAAT	55.0	50.0	58.0	57.9	403 bp	1.0	79.6
rps8-rpl14_p2	ATCCTATCCGAACCGTTCCT	CCCACGGGAATTGAGACAAT	50.0	50.0	57.9	57.9	401 bp	1.0	79.3
rps8-rpl14_p3	TCCTATCCGAACCGTTCCTT	CCCACGGGAATTGAGACAAT	50.0	50.0	58.1	57.9	400 bp	1.0	78.8
rps8-rpl14_p4	CGATCCTATCCGAACCGTTC	AGGAACTCGAGTTTTTGGGG	55.0	50.0	58.0	57.7	427 bp	1.0	78.4
rps8-rpl14_p5	ATCCTATCCGAACCGTTCCT	AGGAACTCGAGTTTTTGGGG	50.0	50.0	57.9	57.7	425 bp	1.0	78.1