Hotspot

rpl20-rps12

LSC · 68463-69226 · 764 bp

Back to Farsetia

Pi 0.00654
MarkerSeek score 62.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
764
variable_sites
5
pi
0.00654
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT ACAGCCAATCCGAAATGTCA 50.0 45.0 58.0 57.8 877-878 bp 1.0 78.6
rpl20-rps12_p2 TTTTGTTCTACGCCTCCGAG ACAGCCAATCCGAAATGTCA 50.0 45.0 57.9 57.8 891-892 bp 1.0 78.3
rpl20-rps12_p3 TCCGAGCTATATATCCGCGT ACAAGACAGCCAATCCGAAA 50.0 45.0 58.0 57.7 882-883 bp 1.0 78.0
rpl20-rps12_p4 TCCGAGCTATATATCCGCGT GAGGAACATGTACTCGGGTG 50.0 55.0 58.0 57.7 821-822 bp 1.0 77.9
rpl20-rps12_p5 TTTTGTTCTACGCCTCCGAG ACAAGACAGCCAATCCGAAA 50.0 45.0 57.9 57.7 896-897 bp 1.0 77.7