Hotspot

trnT-ACA-trnL-UUA

LSC · 45856-46664 · 809 bp

Back to Farsetia

Pi 0.0051
MarkerSeek score 64.70
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
809
variable_sites
4
pi
0.0051
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 GCGATGCTCTAACCTCTGAG ATTAAGTCCGCAGCGTCTAC 55.0 50.0 57.9 57.8 865-877 bp 1.0 80.0
trnT-ACA-trnL-UUA_p2 TATGGAAAAGCCGGCTATCG ATTAAGTCCGCAGCGTCTAC 50.0 50.0 57.8 57.8 916-928 bp 1.0 79.6
trnT-ACA-trnL-UUA_p3 GGAATCGAACCGATGACCAT ATTAAGTCCGCAGCGTCTAC 50.0 50.0 57.8 57.8 897-909 bp 1.0 79.0
trnT-ACA-trnL-UUA_p4 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 890-902 bp 1.0 77.5
trnT-ACA-trnL-UUA_p5 GCGATGCTCTAACCTCTGAG CGTCTACCAATTCCGCCATA 55.0 50.0 57.9 57.5 852-864 bp 1.0 77.5