Hotspot

trnT-ACC-psbD

LSC · 31280-32330 · 1051 bp

Back to Farsetia

Pi 0.00875
MarkerSeek score 56.00
Primer available yes
Label trnT-ACC-psbD

Feature Metadata

label_name
trnT-ACC-psbD
feature_type
igs
region
LSC
length_bp
1051
variable_sites
9
pi
0.00875
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACC-psbD_p1 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1112-1130 bp 1.0 42.3
trnT-ACC-psbD_p2 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1115-1133 bp 1.0 42.3
trnT-ACC-psbD_p3 CCAAGACGGCCCTTTTAACT TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1134-1152 bp 1.0 42.2
trnT-ACC-psbD_p4 ACGGCCCTTTTAACTCAGTG TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1129-1147 bp 1.0 42.2
trnT-ACC-psbD_p5 GACGGCCCTTTTAACTCAGT TTTACCAAGGGCTATAGTCA 50.0 40.0 57.8 53.0 1130-1148 bp 1.0 42.2