Hotspot

petN-psbM

LSC · 28306-28849 · 544 bp

Back to Farsetia

Pi 0.013

MarkerSeek score 66.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 7
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	GCACTCTTCATTCTCGTTCCT	50.0	47.6	58.0	58.0	639-645 bp	1.0	73.3
petN-psbM_p2	CGTAGTGTGGGGAAGAAGTG	GCACTCTTCATTCTCGTTCCT	55.0	47.6	57.9	58.0	638-644 bp	1.0	72.7
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	ACTGCACTCTTCATTCTCGT	50.0	45.0	58.0	56.9	642-648 bp	1.0	72.6
petN-psbM_p4	CGTAGTGTGGGGAAGAAGTG	ACTGCACTCTTCATTCTCGT	55.0	45.0	57.9	56.9	641-647 bp	1.0	72.0
petN-psbM_p5	CTCTCGTAGTGTGGGGAAGA	GCACTCTTCATTCTCGTTCCT	55.0	47.6	58.2	58.0	642-648 bp	1.0	72.0