Hotspot

rpl16

LSC · 80295-81802 · 1508 bp

Back to Fargesia

Pi 0.00199

MarkerSeek score 37.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1508
variable_sites: 17
pi: 0.00199
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TAACCGCTGCATTGTCATCA	GGACTCAGGCTCAAGTTCTG	45.0	55.0	57.9	57.9	2514-2524 bp	1.0	79.5
rpl16_p2	TGATAACCGCTGCATTGTCA	GGACTCAGGCTCAAGTTCTG	45.0	55.0	57.9	57.9	2517-2527 bp	1.0	79.5
rpl16_p3	TCAGCAATAGTGTCCTTGCC	GGACTCAGGCTCAAGTTCTG	50.0	55.0	57.9	57.9	2792-2802 bp	1.0	79.4
rpl16_p4	ACCGTCTTCGCATTTGAACT	GGACTCAGGCTCAAGTTCTG	45.0	55.0	58.1	57.9	2480-2490 bp	1.0	79.4
rpl16_p5	TTTCTAGAGGCATTTGGGGC	GGACTCAGGCTCAAGTTCTG	50.0	55.0	57.9	57.9	2418-2428 bp	1.0	79.2