Hotspot

psbA

LSC · 87-1148 · 1062 bp

Back to Fargesia

Pi 0.000579

MarkerSeek score 33.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.000579
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCTGGCTTTCCTTCCTTCAA	AATACGTGTGCTTGGGAGTC	45.0	50.0	56.7	57.9	1159 bp	0.944444	66.4
psbA_p2	TTCTGGCTTTCCTTCCTTCA	AATACGTGTGCTTGGGAGTC	45.0	50.0	56.7	57.9	1160 bp	0.944444	66.4
psbA_p3	TCTGGCTTTCCTTCCTTCAAA	AATACGTGTGCTTGGGAGTC	42.9	50.0	57.4	57.9	1159 bp	0.944444	64.4
psbA_p4	TTCTGGCTTTCCTTCCTTCAA	AATACGTGTGCTTGGGAGTC	42.9	50.0	57.4	57.9	1160 bp	0.944444	64.4
psbA_p5	TTTCTGGCTTTCCTTCCTTCA	AATACGTGTGCTTGGGAGTC	42.9	50.0	57.4	57.9	1161 bp	0.944444	64.4