Hotspot

atpF

LSC · 35241-36632 · 1392 bp

Back to Fargesia

Pi 0.00129

MarkerSeek score 46.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1392
variable_sites: 11
pi: 0.00129
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAAAAGGGCGAGCGAAGTAA	TTCGGAAGCAAGGGGAAAAA	45.0	45.0	58.1	57.9	1529-1542 bp	1.0	79.5
atpF_p2	AAAAAGGGCGAGCGAAGTAA	TTTCGGAAGCAAGGGGAAAA	45.0	45.0	58.1	57.9	1530-1543 bp	1.0	79.5
atpF_p3	AAAAAGGGCGAGCGAAGTAA	TTTTCGGAAGCAAGGGGAAA	45.0	45.0	58.1	57.9	1531-1544 bp	1.0	79.5
atpF_p4	AAAAAGGGCGAGCGAAGTAA	TTTTTCGGAAGCAAGGGGAA	45.0	45.0	58.1	57.9	1532-1545 bp	1.0	79.5
atpF_p5	AAAAAGGGCGAGCGAAGTAA	TCGAAGGGTTGCCATTAGTG	45.0	50.0	58.1	57.9	1565-1578 bp	1.0	79.3