Hotspot

rpl32-trnL-UAG

SSC · 108408-109109 · 702 bp

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Pi 0.00294
MarkerSeek score 47.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
702
variable_sites
12
pi
0.00294
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 774-777 bp 1.0 79.2
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 773-776 bp 1.0 79.2
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 767-770 bp 1.0 78.8
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 766-769 bp 1.0 78.8
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 58.1 58.2 787-790 bp 1.0 78.7