Hotspot

ndhF-rpl32

SSC · 107339-108227 · 889 bp

Back to Fargesia

Pi 0.00289

MarkerSeek score 54.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 889
variable_sites: 17
pi: 0.00289
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	981-1019 bp	1.0	44.2
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	953-991 bp	1.0	44.0
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	954-992 bp	1.0	44.0
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	955-993 bp	1.0	44.0
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	956-994 bp	1.0	44.0