Hotspot

rpl33-rps18

LSC · 67918-68180 · 263 bp

Back to Fargesia

Pi 0.00413

MarkerSeek score 48.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 263
variable_sites: 8
pi: 0.00413
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.0	58.0	703-728 bp	1.0	81.3
rpl33-rps18_p2	CAAGCTAAGTCAGGCCTCAG	TGACTGGGCTTGAAATTGCT	55.0	45.0	58.0	58.0	1076-1101 bp	1.0	81.1
rpl33-rps18_p3	CATAGAAAGAGGCTGGGCTG	TGACTGGGCTTGAAATTGCT	55.0	45.0	58.0	58.0	1047-1072 bp	1.0	81.0
rpl33-rps18_p4	AGAATCGCCACAATACACCC	CGAAAAGTTTGCTTGGATTTACG	50.0	39.1	58.0	57.5	462-487 bp	1.0	54.1
rpl33-rps18_p5	CCACAATACACCCGGACAAT	CGAAAAGTTTGCTTGGATTTACG	50.0	39.1	57.9	57.5	455-480 bp	1.0	53.4