Hotspot

trnS-GCU-psbD

LSC · 8469-9451 · 983 bp

Back to Fargesia

Pi 0.00172

MarkerSeek score 39.70

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 11
pi: 0.00172
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ACTCTACCAAGGGCTATAGTCA	45.0	45.5	58.0	57.5	1036-1058 bp	1.0	61.1
trnS-GCU-psbD_p2	GCTTTAGTCCACTCAGCCAT	ACTCTACCAAGGGCTATAGTCA	50.0	45.5	57.6	57.5	1022-1044 bp	1.0	58.1
trnS-GCU-psbD_p3	AGTCCACTCAGCCATCTCTC	ACTCTACCAAGGGCTATAGTCA	55.0	45.5	58.5	57.5	1017-1039 bp	1.0	57.3
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	ACTCTACCAAGGGCTATAGTCAT	45.0	43.5	58.0	57.9	1036-1058 bp	1.0	56.7
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	AACTCTACCAAGGGCTATAGTCA	45.0	43.5	58.0	58.1	1037-1059 bp	1.0	56.4