Hotspot

accD

LSC · 60313-61827 · 1515 bp

Back to Fallopia

Pi 0.0364

MarkerSeek score 70.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 105
pi: 0.0364
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CTGTACTACAGTTCGGTGGC	GAATTTGGATGGCAACCACG	55.0	50.0	58.0	58.0	2290 bp	0.25	49.9
accD_p2	AGAGAGTGGAGGGATACACG	GAATTTGGATGGCAACCACG	55.0	50.0	57.9	58.0	1711 bp	0.25	49.5
accD_p3	CACTCAACCTTGGGTTTCCA	GAATTTGGATGGCAACCACG	50.0	50.0	57.9	58.0	2391 bp	0.25	49.4
accD_p4	GCCTGTGTACAAGCTCGTAA	GAATTTGGATGGCAACCACG	50.0	50.0	57.9	58.0	2207 bp	0.25	49.4
accD_p5	GGTTTCCATGCCTGGTGTTA	GAATTTGGATGGCAACCACG	50.0	50.0	58.1	58.0	2379 bp	0.25	49.3