Hotspot

rpoA-rps11

LSC · 83365-83440 · 76 bp

Back to Fallopia

Pi 0.0504

MarkerSeek score 77.50

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 76
variable_sites: 7
pi: 0.0504
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ATCCAGGTCCCCTAACACAA	CTAACGGACTCGATGCACAA	50.0	50.0	57.9	58.0	947 bp	0.25	49.5
rpoA-rps11_p2	ATCCAGGTCCCCTAACACAA	TCGTAAAGTCGTCGAGCAAG	50.0	50.0	57.9	58.0	597 bp	0.25	49.2
rpoA-rps11_p3	ATCCAGGTCCCCTAACACAA	AAGGGATGCAGCATTACGAG	50.0	50.0	57.9	58.0	534 bp	0.25	49.2
rpoA-rps11_p4	ATCCAGGTCCCCTAACACAA	GAAGGGATGCAGCATTACGA	50.0	50.0	57.9	58.0	535 bp	0.25	49.2
rpoA-rps11_p5	ATCCAGGTCCCCTAACACAA	GTAAAGTCGTCGAGCAAGGT	50.0	50.0	57.9	57.9	595 bp	0.25	49.1