Hotspot

trnM-CAU-atpE

LSC · 55486-55752 · 267 bp

Back to Fallopia

Pi 0.0453

MarkerSeek score 75.10

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 21
pi: 0.0453
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	ACACGGGTAGAGGCTATCAA	50.0	50.0	58.1	57.8	330-340 bp	1.0	86.9
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	GACACGGGTAGAGGCTATCA	50.0	55.0	58.1	58.3	331-341 bp	1.0	85.6
trnM-CAU-atpE_p3	TTGCTTTCATACGGCAGGAG	ACACGGGTAGAGGCTATCAA	50.0	50.0	58.3	57.8	342-352 bp	1.0	85.2
trnM-CAU-atpE_p4	CATACGGCAGGAGTCATTGG	ACACGGGTAGAGGCTATCAA	55.0	50.0	58.4	57.8	335-345 bp	1.0	84.2
trnM-CAU-atpE_p5	ATTGCTTTCATACGGCAGGA	ACACGGGTAGAGGCTATCAA	45.0	50.0	57.6	57.8	343-353 bp	1.0	83.9