Hotspot

psaA-ycf3

LSC · 44688-45433 · 746 bp

Back to Fallopia

Pi 0.0366

MarkerSeek score 74.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 50
pi: 0.0366
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGTTTCAGGGCCCTTAGCTA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	938-990 bp	1.0	88.1
psaA-ycf3_p2	TAGTTTCAGGGCCCTTAGCT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	939-991 bp	1.0	88.1
psaA-ycf3_p3	ATGCCACTCAGCCAAAGAAA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.0	58.0	1075-1127 bp	1.0	87.9
psaA-ycf3_p4	GTTATTCCAGAAGCTCGCCA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	1264-1316 bp	1.0	87.2
psaA-ycf3_p5	TAATGGAACCAACCGGCAAA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.0	58.0	1345-1397 bp	1.0	87.1