Hotspot

petN-psbM

LSC · 30284-31377 · 1094 bp

Back to Fallopia

Pi 0.0478

MarkerSeek score 81.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 92
pi: 0.0478
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTGTTCAGTAACCCCGAGT	CGGCGAAGTGATTTGATAGC	50.0	50.0	58.0	57.3	1805-1880 bp	1.0	83.7
petN-psbM_p2	CCTTTCACTCGTCGTATGGG	CGGCGAAGTGATTTGATAGC	55.0	50.0	58.1	57.3	1395-1480 bp	1.0	83.6
petN-psbM_p3	CGTCGTATGGGGAAGAAGTG	CGGCGAAGTGATTTGATAGC	55.0	50.0	58.1	57.3	1386-1471 bp	1.0	83.6
petN-psbM_p4	CTTTCACTCGTCGTATGGGG	CGGCGAAGTGATTTGATAGC	55.0	50.0	58.1	57.3	1394-1479 bp	1.0	83.6
petN-psbM_p5	AATTGCGGGTAAGAATCCCC	CGGCGAAGTGATTTGATAGC	50.0	50.0	57.9	57.3	1558-1643 bp	1.0	83.4