Hotspot

trnC-GCA-petN

LSC · 29427-30193 · 767 bp

Back to Fallopia

Pi 0.0369

MarkerSeek score 78.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 51
pi: 0.0369
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCGGTTCAAATCTGG	CCATACGACGAGTGAAAGGG	50.0	55.0	58.1	58.1	848-868 bp	1.0	86.6
trnC-GCA-petN_p2	TTTCCCCGGTTCAAATCTGG	CCCATACGACGAGTGAAAGG	50.0	55.0	58.1	58.1	849-869 bp	1.0	86.5
trnC-GCA-petN_p3	TTTCCCCGGTTCAAATCTGG	CCCCATACGACGAGTGAAAG	50.0	55.0	58.1	58.1	850-870 bp	1.0	86.5
trnC-GCA-petN_p4	TTTCCCCGGTTCAAATCTGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	803-823 bp	1.0	86.4
trnC-GCA-petN_p5	TTTCCCCGGTTCAAATCTGG	ACGACGAGTGAAAGGGAAAA	50.0	45.0	58.1	57.4	844-864 bp	1.0	82.4