Hotspot

atpH-atpI

LSC · 15100-15775 · 676 bp

Back to Fallopia

Pi 0.0402

MarkerSeek score 74.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 50
pi: 0.0402
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TGCTCGGAATCCACAAACAA	45.0	45.0	58.0	58.0	1264-1282 bp	1.0	85.0
atpH-atpI_p2	TACCTTCTACAGCTTGGCCT	TGCTCGGAATCCACAAACAA	50.0	45.0	58.0	58.0	1347-1365 bp	1.0	84.6
atpH-atpI_p3	CCTCGTATTTTCCCCTCTGC	TGCTCGGAATCCACAAACAA	55.0	45.0	58.0	58.0	1384-1402 bp	1.0	84.5
atpH-atpI_p4	CCTCATCCTCAAATCAGCCC	TATCGTCCTTGGGTTCCCTT	55.0	50.0	58.0	58.0	1523-1534 bp	1.0	84.4
atpH-atpI_p5	CCTCATCCTCAAATCAGCCC	TGCTCGGAATCCACAAACAA	55.0	45.0	58.0	58.0	1623-1634 bp	1.0	84.3