Hotspot

trnS-GCU-trnG-UCC

LSC · 9175-10359 · 1185 bp

Back to Fallopia

Pi 0.0499

MarkerSeek score 76.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1185
variable_sites: 69
pi: 0.0499
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	1007-1308 bp	1.0	59.5
trnS-GCU-trnG-UCC_p2	CGAATGACTCGTACAACGGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	977-1278 bp	1.0	58.9
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	1010-1311 bp	1.0	58.4
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	1009-1310 bp	1.0	58.4
trnS-GCU-trnG-UCC_p5	CGAATGACTCGTACAACGGA	GAACGAATCACACTTTTACCACT	50.0	39.1	57.7	57.3	979-1280 bp	1.0	57.8